Improving three-dimensional human pluripotent cell culture efficiency via surface molecule coating

Human pluripotent stem cells (hPSCs) are ideal “raw materials” for making various human cell types regenerative medicine and needed in large numbers. 3D suspension culturing (e.g., stirred-tank bioreactor or STR), which suspends cultures an agitated medium, has been extensively studied to scale up hPSC production. However, a significant problem with is the uncontrolled spheroid agglomeration. It leads growth arrest, apoptosis, inhomogeneity purity quality. We propose that i) inhibiting adhesion can prevent agglomeration ii) inhibition be achieved via coating spheroids biocompatible anti-adhesion molecules. used PEG-lipids as model molecules successfully demonstrate concept. anchor surface through interactions between their lipid chains membrane lipids. The flexible hydrophilic PEG act dynamic barrier adhesion. showed eliminated agglomeration, leading homogenous size distribution improvements rate volumetric yield. This novel approach expected impact large-scale production significantly. Furthermore, generalized other types.